An Inhibited Dopamine Synthesizing Cell Model of AADC Deficiency
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Keywords

AADC deficiency; dopamine; SH-SY5Y; retinoic acid; L-DOPA

How to Cite

Khalid, M., & Mohd Moklas, M. A. (2019). An Inhibited Dopamine Synthesizing Cell Model of AADC Deficiency. Life Sciences, Medicine and Biomedicine, 3(6). https://doi.org/10.28916/lsmb.3.6.2019.24

Abstract

Introduction: Aromatic L-amino acid decarboxylase deficiency (AADC) is a rare autosomal recessive pediatric neurotransmitter disease. To date it remains poorly understood mainly due to an absence of a disease model. The dopaminergic neuroblastoma cell SH-SY5Y was chosen to develop our AADC deficiency model. These cells are not native dopamine synthesizers. Objective: To develop a dopamine-producing cellular model of AADC deficiency using SH-SY5Y neuroblastoma cells. Methods: Dopamine pathway proteins were identified with Western Blotting. Dopaminergic differentiation was attempted using all-trans retinoic acid (ATRA) with dopamine detection via HPLC-ECD post alumina extraction. Treatment with L-DOPA provided SH-SY5Y with excess precursor. RT-PCR was used to determine the expression of markers of mature neurons. Results: Western Blot screening identified AADC, dopamine β-hydroxylase and tyrosine hyrdoxylase proteins, indicative of a dopaminergic pathway. ATRA was unsuccessful in producing dopamine from the cells. L-DOPA treatment however, generated dopamine first visible as a HPLC-ECD peak 30 minutes post-incubation. Prior to this, SH-SY5Y dopamine synthesis from L-DOPA has never been documented. This de novo synthesis is then inhibited using benserazide to form our AADC deficiency cell model. RT-PCR showed that SH-SY5Y cells express markers of mature neurons in its ‘native’ state and is not affected by L-DOPA and benserazide treatment. This cell model will potentially benefit many areas of AADC deficiency research. Conclusion: SH-SY5Y cells   produced HPLC-ECD measureable amounts of dopamine with the addition of L-DOPA.    Our model of AADC deficiency is generated by quelling the dopamine production with Benserazide.

https://doi.org/10.28916/lsmb.3.6.2019.24
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