TY - JOUR AU - Kaswan, Nur Khalisah AU - Mohd Suhaimi, Nurul Syazwani AU - Mohammed Izham, Noor Aishah AU - Tengku Mohamad, Tengku Azam Shah AU - Sulaiman, Mohd Roslan AU - Perimal, Enoch Kumar PY - 2020/05/07 Y2 - 2024/03/29 TI - Cardamonin inhibits nitric oxide production modulated through NMDA receptor in LPS-induced SH-SY5Y cell in vitro model JF - Life Sciences, Medicine and Biomedicine JA - LSMB VL - 4 IS - 9 SE - DO - 10.28916/lsmb.4.9.2020.58 UR - https://biomescientia.com/index.php/lsmb/article/view/58 SP - AB - <p><strong>Background: </strong>Cardamonin is a naturally occurring chalcone from the <em>Alpinia</em> <em>species. </em>It is known to possess antioxidant and anti-inflammatory properties. Our previous studies have shown that cardamonin has antihyperalgesic and antiallodynic effects on CCI-induced neuropathic pain in mice. Although the evidence of the association between cardamonin and neuropathic pain has been reported in animal studies, specific targets using <em>in vitro</em> models are still lacking. <strong>Objectives/Methods:</strong> This study aims to investigate the effect of cardamonin on nitric oxide production using the LPS-induced neuropathic pain-like SH-SY5Y in vitro model through NMDA receptor expression. <strong>Results:</strong> Cardamonin administration in differentiated SH-SY5Y cells significantly reduced nitric oxide production assessed using Griess reagent. Western blot analysis demonstrated a significant reduction in GluN2B receptor expression in the cardamonin treated SH-SY5Y cells compared to the vehicle treated group. <strong>Conclusions:</strong> These data suggest that cardamonin reduces nitric oxide production modulated through NMDA GluN2B receptor subunit. Our results provides preliminary data to support the <em>in vivo </em>studies using cardamonin and may contribute to further understanding the mechanisms of action of cardamonin. </p> ER -